England Women will be without injured trio Lauren James, Ella Toone and Lauren Hemp when they face Emma Hayes’ USA side at Wembley on November 30.
Manchester City announced that Hemp had undergone knee surgery on Tuesday, while Toone and James are both suffering with calf issues.
Chelsea’s Niamh Charles and Aston Villa defender Lucy Parker are also sidelined.
Sarina Wiegman has therefore handed Manchester City’s Laura Blindkilde Brown and Leicester City’s Ruby Mace, both 21, their first senior international call-ups.
Meanwhile, Manchester United defender Gabby George, who suffered an ACL injury last year, has returned to the fold for the first time since November 2022.
The 24-player squad will meet at St George’s Park next week before hosting the USA at Wembley later this month and Euro 2025 hosts Switzerland on December 3 at Bramall Lane.
Lauren Hemp’s absence is a significant loss for England as they prepare to host USA
England squad in full
Goalkeepers: Mary Earps (PSG), Hannah Hampton (Chelsea), Anna Moorhouse (Orlando Pride).
Defenders: Lucy Bronze (Chelsea), Millie Bright (Chelsea), Jess Carter (Gotham), Alex Greenwood (Manchester City), Leah Williamson (Arsenal), Esme Morgan (Washington Spirit), Millie Turner (Manchester United), Gabby George (Manchester United), Maya Le Tissier (Manchester United).
Midfielders: Grace Clinton (Manchester United), Fran Kirby (Brighton), Jess Park (Manchester City), Laura Blindkilde Brown (Manchester City), Georgia Stanway (Bayern Munich), Keira Walsh (Barcelona). Ruby Mace (Leicester).
Forwards: Aggie Beever-Jones (Chelsea), Chloe Kelly (Manchester City), Beth Mead (Arsenal), Alessia Russo (Arsenal), Jess Naz (Tottenham).
